Part:BBa_K812014:Design

Auxin production device for expression in tadpole

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1816
  Illegal BamHI site found at 2761
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1021
  Illegal NgoMIV site found at 1523
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been assembled using golden gate technique. The biobrick site are a bit different than the usual one but can still be assembled using the RFC 10 system.

Source

The different protein has been amplified from the bacterial auxin production device of iGEM Imperial 2011 K515100